Calibrations
Submitted by staff on Fri, 2005-06-03 15:04
Note: Confocal images captured at resolution other than 512x512 should be multiplied or divided by the pixel value by the percent resolution difference. Example: 640x640 image captured on the FluoView 1000 upright. That is a 25% increase from 512, so multiply 1.25 (percentage) by 68 pixels (10x objective conversion value). Therefore, 85 pixels equal 100 microns.
| Confocal 1000 Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | FluoView 1000 Upright Confocal Scale Bar Chart (chart assumes image size was not changed from default of 640x640, also that a zoom of 1 was used) | ||||||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||||||
| Objective | Scale Bar | Objective | Scale Bar | ||||
| 4x | 60 pixels = 250 microns | 10x | 50 pixels = 100 microns | ||||
| 10x | 60 pixels = 100 microns | 20x oil | 100 pixels = 100 microns | ||||
| 20x | 60 pixels = 50 microns | 40x oil | 200 pixels = 100 microns | ||||
| 40x | 60 pixels = 25 microns | 40x water | 200 pixels = 100 microns | ||||
| 60x oil | 75 pixels = 20 microns | 60x oil | 300 pixels = 100 microns | ||||
| 60x water | 300 pixels = 100 microns |
|
|||||
| FluoView 300 Custom MultiPhoton Confocal Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | FluoView 1000 Inverted Confocal Scale Bar Chart (chart assumes image size was not changed from default of 640x640, also that a zoom of 1 was used) | ||||||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||||||
| Objective | Scale Bar | Objective | Scale Bar | ||||
| 4x | 20 pixels = 100 microns | ||||||
| 10x | 71 pixels = 100 microns | 10x | 50 pixels = 100 microns | ||||
| 20x water | 145 pixels = 100 microns | 20x oil | 100 pixels = 100 microns | ||||
| 40x water | 290 pixels = 100 microns | 40x oil | 200 pixels = 100 microns | ||||
| 60x oil | 434 pixels = 100 microns | 60x oil | 300 pixels = 100 microns | ||||
|
|
|||||||
| Factoring Scales for Metamorph I (Olympus B-Max 06 Microscope - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | Factoring Scales for Metamorph I (Olympus B-Max 06 Microscope, Scion Camera - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | ||||||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||||||
| Objective | Scale Bar | Objective | Scale Bar | ||||
| 4x | 440 pixels = 100 microns | ||||||
| 10x | 60 pixels = 100 microns | 10x | 111 pixels = 100 microns | ||||
| 20x | 60 pixels = 50 microns | 20x | 225 pixels = 100 microns | ||||
| 40x | 60 pixels = 25 microns | 40x | 450 pixels = 100 microns | ||||
| 60x oil | 75 pixels = 20 microns | 60x water | 682 pixels = 100 microns | ||||
|
|
|||||||
| Factoring Scales for Zeiss (Zeiss Microscope, SPOT camera - chart assumes image size was not changed from default of 1315x1033, also that a zoom of 1 was used) | Inverted and PixCell LCM (Olympus Microscope, DVC camera - chart assumes image size was not changed from default, also that a zoom of 1 was used) | ||||||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||||||
| Objective | Scale Bar | Objective | Scale Bar | ||||
| 2.5x | 25 pixels = 100 microns | 2x | 31 pixels = 100 microns | ||||
| 4x | 38 pixels = 100 microns | 4x | 62 pixels = 100 microns | ||||
| 10x | 95 pixels = 100 microns | 10x | 155 pixels = 100 microns | ||||
| 20x | 190 pixels = 100 microns | 20x | 308 pixels = 100 microns | ||||
| 40x | 190 pixels = 50 microns | 40x | 306 pixels = 50 microns | ||||
| 63x oil | 119 pixels = 20 microns | 60x oil | 187 pixels = 20 microns | ||||
