Purpose:

• Collect optical sections from fluorescent samples with higher z resolution less likelihood of photo damage to live tissue, and greater penetration into sample. (up to 200 microns depending on sample)
• Optical sections can be used for 3-D reconstruction or for in focus infrormation at several optical depths.
• Collect optical sections with water immersion lenses from petri dishes, culture plates, etc.
• Can also be used as a single photon confocal.
• Environmental chamber, micromanipulator and microinjection unit on microscope.
• Samples can be heated and flow-through systems available.
• DIC/Phase and fluorescence can be collected at the same time.
• UV dyes on samples can be used (INDO dyes for example) and calcium flux can be followed.
• Instrument set-up for time series (4-D series).
• Photo flashing/bleaching also available with existing tsunami laser.

Equipment Specifications:

• Tsunami Titanium Sapphire Tunable Solid State Laser on Bio-Rads Multi-Photon System
• Olympus B-Max 50 WI upright fixed stage microscope
• Water immersion lenses
• Micromanipulator
• Microinjection unit

Rental Cost:
$51.00/hr, 25% discount 6:00 p.m - 6:00 a.m.

Reserve time on this equipment

The new Olympus FluoView FV1000 is the latest in point-scanning, point-detection, confocal laser scanning microscopes designed for today's intensive and demanding biological research investigations. Excellent resolution, bright and crisp optics, and high efficiency of excitation, coupled to an intuitive user interface and affordability are key characteristics of this state-of-the-art optical microscopy system.

When configured for visible excitation, the FluoView FV1000 is capable of simultaneous collection from up to 4 detection channels using multiple laser and conventional illumination sources. Wavelength selection and flexible control of laser intensity with advanced AOTF technology permits reduced specimen fading, lower crosstalk, and excitation area selection. Olympus confocal microscopes are ideal for 3-D imaging, time course experiments, energy transfer visualization, and photobleaching recovery experiments.

Rental Cost:
$51.00/hr, 25% discount 6:00 p.m - 6:00 a.m.

Reserve time on this equipment

The new Olympus FluoView FV1000 is the latest in point-scanning, point-detection, confocal laser scanning microscopes designed for today's intensive and demanding biological research investigations. Excellent resolution, bright and crisp optics, and high efficiency of excitation, coupled to an intuitive user interface and affordability are key characteristics of this state-of-the-art optical microscopy system.

When configured for visible excitation, the FluoView FV1000 is capable of simultaneous collection from up to 4 detection channels using multiple laser and conventional illumination sources. Wavelength selection and flexible control of laser intensity with advanced AOTF technology permits reduced specimen fading, lower crosstalk, and excitation area selection. Olympus confocal microscopes are ideal for 3-D imaging, time course experiments, energy transfer visualization, and photobleaching recovery experiments.

Rental Cost:
$51.00/hr, 25% discount 6:00 p.m - 6:00 a.m.

Reserve time on this equipment